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pan t cell isolation kit human (Miltenyi Biotec)

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Miltenyi Biotec pan t cell isolation kit human
Pan T Cell Isolation Kit Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 2794 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pan+t+isolation+kit/Pan+T+Cell+Isolation+Kit%2C+human/pm42898331-190-104-110
Average 97 stars, based on 2794 article reviews

pan t cell isolation kit human - by Bioz Stars, 2026-10

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Article Title: Human kallikrein 2: A novel lineage-specific surface target in prostate cancer
Article Snippet: Leukopaks from healthy male donors were purchased from StemExpress and shipped at room temperature prior to processing and T-cell isolation. .. T cells were isolated using Pan-T isolation kit (Miltenyi 130- 096-535) on the CliniMACS Prodigy system (Miltenyi, RRID:SCR_020289). .. Cells were resuspended in CryoStor-10 (Biolife Systems 210102), aliquoted, frozen, and stored in liquid nitrogen until used in assays.

Article Title: Discovery of a new anti-γc antibody in clinical development for the treatment of autoimmune diseases
Article Snippet: Autoimmune disease refers to a condition when the immune system anomalously attacks its own body and healthy cells.. Although the exact causes of autoimmune diseases are unknown, it is recognized that excessive or aberrant cytokine responses contribute significantly to the development of autoimmunity.. Among them, the common gamma c chain (γc) cytokines driven signaling cascade plays an indispensable role in driving pathogenic immune responses in patients with autoimmune diseases.

Article Title: HPK1 inhibitors and uses thereof
Article Snippet: .. Briefly, pan T cells can be isolated from peripheral blood (PB) mononuclear cells (MNCs), or PBMC, using commercially available kits, such as MACS (Miltenyl Biotec) Pan T isolation kit (Cat. No. 130-096-535). ..

Article Title: CD20+ T follicular helper-like cells drive antigen-specific autoimmunity in bullous pemphigoid
Article Snippet: Following 7 days of700 incubation, cells were harvested for the analysis of B-cell differentiation, and culture supernatants701 were collected for the measurement of antibody titers by enzyme-linked immunosorbent assay.702 703 32 Cell culture and lentiviral transduction704 For PAX5 overexpression, lentiviral constructs carrying the PAX5 gene or a negative control705 sequence were generated and packaged by Tsingke Biotechnology. .. Human primary T cells were706 extracted from PBMCs using a Pan T isolation kit (Miltenyi Biotec, 130-096-535). .. First, T cells707 (2.0 × 105 per well) were activated with anti-CD3/CD28 Dynabeads at a 1:1 ratio in 500 μL of708 RPMI 1640 medium supplemented with 10% FBS and human rIL-2 (30 IU/mL) in 48-well plates.709 After 24 h, 2 × 107 TU of ZsGreen-Puro/CMV-PAX5 or negative control lentivirus in complete710 RPMI 1640 supplemented with rIL-2 and 8 μg/mL polybrene were added to the cell suspension at711 a final volume of 750 μL at a multiplicity of infection (MOI) of 100 for 12 h. Then, the cells were712 harvested and incubated in RPMI 1640 culture medium supplemented with 10% FBS, antibiotics,713 rhIL-2 and anti-CD3/CD28 Dynabeads in 48-well plates for 72 h. To inhibit promoter methylation714 in T cells, a 10 μM concentration of the DNMT inhibitor decitabine (MedChemExpress, HY-715 A0004) was used to pretreat the culture system.

Article Title: Small molecule BCL6-inhibition suppresses follicular T helper cell differentiation and plasma blast formation.
Article Snippet: .. T cells were isolated using an AutoMacsTM and pan-T isolation kit (Miltenyi Biotec), and stimulated with 2 μg/ml of PHA (Sigma-Aldrich) for 24 h, followed by 6 days of stimulation with 80 U/ml of IL-2 (R&D systems). ..

Article Title: Tunneling CARs: Increasing CAR T tumor infiltration through the overexpression of MMP7 and SPP1
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were then isolated by density gradient centrifugation using Lymphoprep solution (Stemcell Technologies, #07801). .. In experiments using freshly-isolated T cells (FI-T), T cells were isolated from PBMCs by negative selection using Miltenyi Biotec’s Pan T isolation kit (#130-096-535) per the manufacturer’s instructions and used immediately. ..

Article Title: Galectin-3 secreted by triple-negative breast cancer cells regulates T cell function.
Article Snippet: Triple-negative breast cancer (TNBC) is an aggressive subtype that accounts for 10-15 % of breast cancer.. Current treatment of high-risk early-stage TNBC includes neoadjuvant chemo-immune therapy.. However, the substantial variation in immune response prompts an urgent need for new immune-targeting agents.

Article Title: Galectin-3 secreted by triple-negative breast cancer cells regulates T cell function
Article Snippet: Recombinant Gal-3 (rGal-3) protein at 2μg/ml (R&D Systems, USA) was added to the Gal-3 neg clones. .. T cells were isolated by negative selection using the Pan T isolation kit (Miltenyi Biotec, USA) following the manufacturer's instructions. ..

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Tumor growth after depletion of CD8⁺ T-cells. A CD8⁺ T-cells were depleted in wildtype and IKKε KO mice by intraperitoneal injection of anti-mouse CD8α as indicated. IgG injection served as isotype control. B Successful depletion was verified by FACS analysis of spleen and tumor samples. The plot shows a representative example depicting CD4, CD8, and double-negative (DN; CD3⁺, CD4⁻, CD8⁻) T-cell populations. C Tumor growth kinetics for each individual mouse over a 21-day period. D Left panel: tumor growth kinetics including only mice that survived at least until day 16. Two-way ANOVA with Tukey’s multiple-comparisons test was applied. Arrows indicate tumor volumes from isotype-control–treated mice. Right panel: tumor mass, analyzed by one-way ANOVA with Tukey’s post hoc test. Group sizes: all mice: WT + isotype control, n = 6; WT + anti-CD8α, n = 6; IKKε-KO + isotype control, n = 5; IKKε-KO + anti-CD8α, n = 6.; mice that survived until day 16: WT + isotype control, n = 5; WT + anti-CD8α, n = 3 mice; IKKε-KO + isotype control, n = 4; IKKε-KO + anti-CD8α, n = 5

Journal: Molecular Medicine

Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

doi: 10.1186/s10020-026-01651-w

Figure Lengend Snippet: Tumor growth after depletion of CD8⁺ T-cells. A CD8⁺ T-cells were depleted in wildtype and IKKε KO mice by intraperitoneal injection of anti-mouse CD8α as indicated. IgG injection served as isotype control. B Successful depletion was verified by FACS analysis of spleen and tumor samples. The plot shows a representative example depicting CD4, CD8, and double-negative (DN; CD3⁺, CD4⁻, CD8⁻) T-cell populations. C Tumor growth kinetics for each individual mouse over a 21-day period. D Left panel: tumor growth kinetics including only mice that survived at least until day 16. Two-way ANOVA with Tukey’s multiple-comparisons test was applied. Arrows indicate tumor volumes from isotype-control–treated mice. Right panel: tumor mass, analyzed by one-way ANOVA with Tukey’s post hoc test. Group sizes: all mice: WT + isotype control, n = 6; WT + anti-CD8α, n = 6; IKKε-KO + isotype control, n = 5; IKKε-KO + anti-CD8α, n = 6.; mice that survived until day 16: WT + isotype control, n = 5; WT + anti-CD8α, n = 3 mice; IKKε-KO + isotype control, n = 4; IKKε-KO + anti-CD8α, n = 5

Article Snippet: CD3 T-cells were isolated from the spleen of wildtype and IKKε KO mice using a Pan T-cell Isolation Kit II, mouse, (Miltenyi Bergisch Gladbach, Germany, #130–095–130) and directly used without prior activation with CD3/CD28 Beads.

Techniques: Injection, Control, Analysis

Epidemiology and expression of IKKε in human skin cancer. A Survival proportions of skin cancer patients in relation to IKKε expression levels, adapted from the Human Protein Atlas ( https://www.proteinatlas.org/).The cut-off IKKε expression was calculated by X-tile optimization. * P < 0.05, significant difference between low and high IKKε expression. B Multiplex immunofluorescence analysis showing protein expression of IKKε (red), tumor (S100, blue) and T-cells (CD3 + , green; CD4 + , yellow; CD8⁺, turquoise; γδ-T-cells, orange) in human primary melanoma (representative picture from n = 9 patient samples), Scale Bars: 50 µm. C Quantitative analysis of tumor cells (S100⁺) and CD3⁺ T cells, including colocalization of IKKε expression within tumor cells and CD3⁺ T cells. Values are presented as the percentage of S100⁺ or CD3⁺ cells normalized to the total cell count, or as the proportion of IKKε-positive cells relative to the total number of analyzed cells within the cell type (tumor cells or CD3⁺ T cells), respectively. D Quantitative analysis of T-cell subsets in human melanoma samples. Values are shown as percentages relative to the CD3⁺ T-cell population. Horizontal lines indicate the median. Each dot represents one patient sample ( n = 9). For each sample, 3–8 representative fields of view were analyzed

Journal: Molecular Medicine

Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

doi: 10.1186/s10020-026-01651-w

Figure Lengend Snippet: Epidemiology and expression of IKKε in human skin cancer. A Survival proportions of skin cancer patients in relation to IKKε expression levels, adapted from the Human Protein Atlas ( https://www.proteinatlas.org/).The cut-off IKKε expression was calculated by X-tile optimization. * P < 0.05, significant difference between low and high IKKε expression. B Multiplex immunofluorescence analysis showing protein expression of IKKε (red), tumor (S100, blue) and T-cells (CD3 + , green; CD4 + , yellow; CD8⁺, turquoise; γδ-T-cells, orange) in human primary melanoma (representative picture from n = 9 patient samples), Scale Bars: 50 µm. C Quantitative analysis of tumor cells (S100⁺) and CD3⁺ T cells, including colocalization of IKKε expression within tumor cells and CD3⁺ T cells. Values are presented as the percentage of S100⁺ or CD3⁺ cells normalized to the total cell count, or as the proportion of IKKε-positive cells relative to the total number of analyzed cells within the cell type (tumor cells or CD3⁺ T cells), respectively. D Quantitative analysis of T-cell subsets in human melanoma samples. Values are shown as percentages relative to the CD3⁺ T-cell population. Horizontal lines indicate the median. Each dot represents one patient sample ( n = 9). For each sample, 3–8 representative fields of view were analyzed

Techniques: Expressing, Multiplex Assay, Immunofluorescence, Analysis, Cell Characterization

Tumor growth, immune cell composition and CD3 T-cell immunofluorescence. A Tumor growth in wildtype (WT) and IKKε KO mice over a period of 21 days and tumor masses at the end of the experiment. WT: n = 13 mice, IKKε KO: n = 15 mice, Two-way Anova, Tukey´s multiple comparisons test; tumor mass, Mann Whitney test. B Immune cell numbers in the tumors as determined by flow cytometry. Cell counts were normalized to the number of living CD45 + immune cells. n = 6/group; Student´s t-test. C Immunofluorescence analysis of CD3-positive cells in the tumor. Nuclei were stained with DAPI and CD3 signals normalized to the cell number. Scale Bar represents 100 µm. Representative figure from WT n = 7, and IKKε KO n = 6, the diagram shows the quantitative analysis of all tumors analyzed. Student´s t-test. * P < 0.05, ** P < 0.01, *** P < 0.001

Journal: Molecular Medicine

Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

doi: 10.1186/s10020-026-01651-w

Figure Lengend Snippet: Tumor growth, immune cell composition and CD3 T-cell immunofluorescence. A Tumor growth in wildtype (WT) and IKKε KO mice over a period of 21 days and tumor masses at the end of the experiment. WT: n = 13 mice, IKKε KO: n = 15 mice, Two-way Anova, Tukey´s multiple comparisons test; tumor mass, Mann Whitney test. B Immune cell numbers in the tumors as determined by flow cytometry. Cell counts were normalized to the number of living CD45 + immune cells. n = 6/group; Student´s t-test. C Immunofluorescence analysis of CD3-positive cells in the tumor. Nuclei were stained with DAPI and CD3 signals normalized to the cell number. Scale Bar represents 100 µm. Representative figure from WT n = 7, and IKKε KO n = 6, the diagram shows the quantitative analysis of all tumors analyzed. Student´s t-test. * P < 0.05, ** P < 0.01, *** P < 0.001

Techniques: Immune Cell, Immunofluorescence, MANN-WHITNEY, Flow Cytometry, Analysis, Staining

T-cell migration and activity. A T-cell migration towards a FCS gradient or conditioned medium from B16BL6 melanoma cells. Pan T-cells were isolated from the spleens of wildtype (WT) and IKKε KO mice and plated into cell culture insert filled with T-cell medium containing 0.1% FCS. The bottom chamber was filled either with medium containing 0.1% FCS (neg ctrl), 10% FCS (pos ctrl) or conditioned medium from B16BL6 cells without FCS (B16). Cell migration of different T-cell subtypes to the lower channel was determined by FACS analysis. Cell numbers were normalized to counting beads and negative controls. n = 3–6. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, ** P < 0.01. B — D Effects of co-culture of B16BL6 melanoma cells with CD8⁺ and γδ-T-cells, respectively, derived from the spleens of wildtype (WT) and IKKε KO mice. B Cell proliferation determined by WST assay, B16BL6 monocultures were set as 1 for better comparison. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, **P < 0.01 significant difference in comparison to B16BL6 monoculture ( C ) IFN-γ levels analyzed by cytokine bead array (CBA) and ( D ) granzyme B release determined by ELISA. n = 4–10/group. C and D The T-cell monocultures of wildtype and IKKε knock-out mice were set as 1 for better comparison. Student´s t-test, * P < 0.05, significant difference in comparison to wildtype, # P < 0.05, ## P < 0.01, ### P < 0.001 significant difference in comparison to T-cell monocultures

Journal: Molecular Medicine

Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

doi: 10.1186/s10020-026-01651-w

Figure Lengend Snippet: T-cell migration and activity. A T-cell migration towards a FCS gradient or conditioned medium from B16BL6 melanoma cells. Pan T-cells were isolated from the spleens of wildtype (WT) and IKKε KO mice and plated into cell culture insert filled with T-cell medium containing 0.1% FCS. The bottom chamber was filled either with medium containing 0.1% FCS (neg ctrl), 10% FCS (pos ctrl) or conditioned medium from B16BL6 cells without FCS (B16). Cell migration of different T-cell subtypes to the lower channel was determined by FACS analysis. Cell numbers were normalized to counting beads and negative controls. n = 3–6. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, ** P < 0.01. B — D Effects of co-culture of B16BL6 melanoma cells with CD8⁺ and γδ-T-cells, respectively, derived from the spleens of wildtype (WT) and IKKε KO mice. B Cell proliferation determined by WST assay, B16BL6 monocultures were set as 1 for better comparison. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, **P < 0.01 significant difference in comparison to B16BL6 monoculture ( C ) IFN-γ levels analyzed by cytokine bead array (CBA) and ( D ) granzyme B release determined by ELISA. n = 4–10/group. C and D The T-cell monocultures of wildtype and IKKε knock-out mice were set as 1 for better comparison. Student´s t-test, * P < 0.05, significant difference in comparison to wildtype, # P < 0.05, ## P < 0.01, ### P < 0.001 significant difference in comparison to T-cell monocultures

Techniques: Migration, Activity Assay, Isolation, Cell Culture, Analysis, Counting, Co-culture Assay, Derivative Assay, WST Assay, Comparison, Enzyme-linked Immunosorbent Assay, Knock-Out

Proteomic analysis of B16BL6 cells with and without co-culture with WT or IKKε KO CD8⁺ T-cells. B16BL6 were either cultured as monoculture or as co-culture with CD8⁺ T-cells from wildtype and IKKε KO mice. Resulting protein lysates were subjected to proteome analysis by LC-MSMS. A Venn diagram, PCA and Volcano plots showing apparent differences between mono- and co-culture as well as proteins regulated by wildtype or IKKε KO T-cells, respectively. B Gene ontology analysis of differentially regulated proteins. Proteins involved in the significant regulation of the inflammatory response in co-culture of B16BL6 with IKKε KO T-cells are indicated in the respective Volcano plot. n = 5 B16BL6 monoculture-and n = 4 for B16BL6-T-cell co-cultures

Journal: Molecular Medicine

Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

doi: 10.1186/s10020-026-01651-w

Figure Lengend Snippet: Proteomic analysis of B16BL6 cells with and without co-culture with WT or IKKε KO CD8⁺ T-cells. B16BL6 were either cultured as monoculture or as co-culture with CD8⁺ T-cells from wildtype and IKKε KO mice. Resulting protein lysates were subjected to proteome analysis by LC-MSMS. A Venn diagram, PCA and Volcano plots showing apparent differences between mono- and co-culture as well as proteins regulated by wildtype or IKKε KO T-cells, respectively. B Gene ontology analysis of differentially regulated proteins. Proteins involved in the significant regulation of the inflammatory response in co-culture of B16BL6 with IKKε KO T-cells are indicated in the respective Volcano plot. n = 5 B16BL6 monoculture-and n = 4 for B16BL6-T-cell co-cultures

Techniques: Analysis, Co-culture Assay, Cell Culture, Co-Cultures

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